glutathione charge at high ph The System: A Journey from Cyanobacteria to Higher Eukaryotes Number of Tests:100 Assays

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Number of Tests:100 Assays

(8)制备Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂： (8) Preparation of Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: 在步骤(7)的Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂中，加入脱帽试剂10～50℃反应5～30分钟，用真空泵抽干，重新加入脱帽试剂10～50℃反应10～60分钟，抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，加入用DMF溶解的Fmoc-Pro-OH、DIEA、TBTU/HOBt或HBTU/HOBt或BOP/HOBt的混合物，10～50℃反应10～60分钟，用真空泵抽干，用异丙醇洗涤3次，DMF洗涤3次，重蒸过的DMF洗涤2次，抽干，获得Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-树脂； In the Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin of step (7), add decapping reagent 10～50 ℃ and react for 5～30 minutes, dry with vacuum pump, re- Add capping reagent and react at 10-50°C for 10-60 minutes, pump dry, wash with isopropanol for 3 times, DMF for 3 times, distilled DMF for 2 times, add Fmoc-Pro-OH and DIEA dissolved in DMF , a mixture of TBTU/HOBt or HBTU/HOBt or BOP/HOBt, react at 10-50°C for 10-60 minutes, dry it with a vacuum pump, wash 3 times with isopropanol, wash 3 times with DMF, wash 2 times with re-distilled DMF time, drained to obtain Fmoc-Pro-Ala-Asp (otBu)-Asp (otBu)-Ala-Gly-Leu-Val-resin