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Serving as a vital component of mitochondrial functions and ATP synthesis, PQQ functions as a cofactor for several redox activated enzymes (Cores et al., 2023)

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The membrane was washed three times with TBST (0.1% Tween) buffer, sealed with protein-free rapid closure solution (Shanghai Baibo Biotechnology Co., Ltd.) at room temperature for 30 min, washed three times again and then incubated with the primary antibodies on a shaker at 4°C overnight