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For example, oral administration of heat-treated Lactobacillus plantarum OLL2712 prevented the downregulation of ZO-1 and occludin in HFD-fed mice, promoting intestinal barrier integrity (Wang et al., 2023)
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Plates were preincubated with the covalent probe or DMSO for 15 min at 25 °C, followed by 60 min incubation at 25 °C with the fluorescent tracer ( 24 ) and then FP was measured on a SpectraMax iD5 plate reader with fluorescence polarization readout (λ ex = 540 nm, λ em = 590 nm) and reported as milli-polarization units (mP)
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