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Upon receipt, systems were maintained, for 2–7 days prior to use, at the ALI in modified 6-well hanging top plates with 5 mL of EpiAlveolar™ culture medium (MatTek) in the basolateral compartment and 75 µL of media on the apical surface and incubated at standard culture conditions (37°C ± 1°C in a humidified atmosphere of 5% CO 2 in air)
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