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Because of the potential for a fully microbial-based ascorbate-manufacturing process to replace the largely chemical Reichstein process, the company Bio-Technical Resources (Wisconsin, USA) had been investigating ascorbate production in the green alga Chlorella and then in a heterotrophic relative Prototheca moriformis , filing a patent application in 1999 proposing a very similar pathway to the d-Man/l-Gal pathway (Berry et al