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calorie restriction alone Subcutaneous fat: reduction of 40–45% (less than visceral fat) Lean body mass: preservation 95-98% (no catabolic effects) Genetic Obesity Models (ob/ob, db/db): Body weight: 25–30% reduction in ob/ob mice (8 weeks) Leptin: partial normalization of circulating levels Adiponectin: increase of 150–200% Inflammatory profile: reduced levels of IL-6, TNF-α, and MCP-1 (40–60%) Molecular Lipolysis: Triglyceride mobilization: release of free fatty acids +180% Plasma glycerol: increased 150% (lipolysis marker) HSL phosphorylation: increased enzymatic activity 200% ATGL (adipose triglyceride lipase): upregulation 80% Human Clinical Studies (Phase 2): Weight loss: 2.6 kg additional vs placebo (12 weeks, 300 μg/day) Abdominal fat: circumference reduction 4.1 cm vs 1.9 cm placebo IGF-1-free: stable levels (growth safety) Glucose: improves oral tolerance without hypoglycemia 2